According to the critical parameters of sample selection described in this study, and using different culture media proposed to enhance the growth of mesenchymal stem cells (MSC), in parallel with the use of different methods of cell isolation, the researchers were not able to establish MSC cultures from more than one out of 15 UCB samples. Given the high frequency of MSC in UCM, the study hypothesizes that there may be MSC contamination while collecting cord blood. This may explain the rare described cases where MSC isolation from UCB has been possible. However, it could not be ascertained whether the collection method may have caused the disappearance of circulating MSC from the cord blood MNC compartment in favor of the endothelial/subendothelial layer of the UCM. They conclude that UCB can be excluded as a reliable source of MSC in favor of the richer and more reproducible source that is the UCM, meaning the umbilical cord matrix (UCM) is a better source of mesenchymal stem cells (MSC) than the umbilical cord blood (UCB).
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